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W el co m e t o MI NE -L a b@ City U !
Multiscale Integrative NeuroEngineering Laboratory
12/22/2017, Congratulations to Lin Xudong, our manuscript entitled “Core–Shell–Shell
Upconversion Nanoparticles with Enhanced Emission for Wireless Optogenetic Inhibition
is accepted for publication on Nano Letters.
12/12/2017, Congratulations to Xu Zhen, our manuscript entitled “Regeneration of
Cortical Tissue from Brain Injury by Implantation of Defined Molecular Gradient of
Semaphorin 3A” is accepted for publication on Biomaterials.
12/04/2017, Congratulations to Lin Xudong, he won the 2017 graduate student award
from Hong Kong Medical and Healthcare Device Industries Association (HKMHDIA) for his
great work during his PHD studies.
11/30/2017, Congratulations to Zhang Wencong for his successful thesis defense.
11/07/2017, Congratulations to Xu Bingzhe for his successful thesis defense.
10/30/2017, Congratulations to Wang Ying for her successful thesis defense.
10/10/2017, Congratulations to Xu Zhen for his successful thesis defense.
07/15/2017, Congratulations to Lin Xudong, our manuscript entitled “Multiplexed
Optogenetic Stimulation of Neurons with Spectrum-Selective Upconversion Nanoparticles
is accepted for publication on Advanced Healthcare materials.
07/01/2017, Congratulations to Wang Ying, our manuscript entitled “Tetherless Near-
infrared Control of Brain Activity in Behaving Animals Using Fully Implantable
Upconversion Microdevices” is accepted for publication on Biomaterials.
02/24/2017, Congratulations to Lin Xudong for his successful thesis defense.
01/31/2017, Congratulations to Xu Bingzhe, our manuscript entitled “Cell Generator: A
Self-sustaining Bio-hybrid System Based on Energy Harvesting from Engineered Cardiac
Micro-tissuesis accepted for publication on Advanced Functional materials.
12/01/2016, Congratulations to Li Wei, he won the 2016 graduate student award from
Hong Kong Medical and Healthcare Device Industries Association (HKMHDIA) for his
great work during his PHD studies.
02/01/2016, Congratulations to Bingzhe, our manuscript entitled “Ultrathin Mxene-
micropattern-based Field-effect Transistor for Probing Neural Activity ” has been accepted
for publication on Advanced Materials. [link]
12/20/2015, Welcome Luo Xuan joining MINE-Lab.
09/07/2015, Congratulations to Zixun, our manuscript entitled “Interrogation of Cellular
Innate Immunity by Diamond-nanoneedle-assisted Intracellular Molecular Fishing” has
We are actively looking for students from a wide range of backgrounds
(engineering, bio-photonics, material science, and neuroscience) with ingenuity,
productivity, and ability to work in a diverse, high energy environment. Please
send your current CV/resume to pengshi@cityu.edu.hk
Perspective students should also note the deadlines for application to CityU, and
please do check the Hong Kong fellowship scheme as well:
http://www.cityu.edu.hk/mbe/prg-phdmphil.htm
http://www.sgs.cityu.edu.hk/prospective/rpg/hkphd#13
The research efforts in our group focus on the integration of different engineering
principles (electrical, mechanical, optical and information technology) to solve biomedical
problems, especially in neuroscience related areas. The ongoing projects mainly included
on the design, fabrication, and use of advanced macro/micro/nano-biosystems for (1) the
development of high-throughput drug/genetic screening technologies, (2) the study of
novel biomaterials for neuroengineering applications, (3) the development of biohybrid
system based on neural networks of different scales.
City Unive rsity of Hong Kong
香港城市大
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Micro/Nano System based Neural Interface
As the current paradigms of drug discovery evolve, it is vital to have a comprehensive
understanding of the interactions between pharmaceuticals and organismal biology.
We are developing highly transformative technologies based on zebrafish model to
enable functional analysis of neuropharmacology. In combination with information
technology and big data analysis, the goal of the project is to provide a new strategy
as a shortcut for drug discovery, or for repositioning the currently used drugs for
treating neurological diseases.
High-throughput whole-organism screening technology
The ability of neural tissues to carry out complex and concurrent processes results
from the organization of connections between neurons inherent in these structures.
The capability to modulate neural processes at the different levels is a crucial step in
the understanding of the overall neural function. Using microfabrication, we are
developing innovative technologies to address this challenge.
Novel materials/structures/strategies are designed and implemented for regenerating
neural tissue from diseases and injuries. As exemplified by the above pictures,
recreation of exogenous Sema3A gradient in an injured adult rat brain can induce the
migration of endogenous neural progenitor cells for brain tissue regeneration.
Neural Tissue Regeneration
City Univ. | CityU Engineering | CityU MBE |
The interaction between live biological organisms and the man-made machines is bi-
directional. Different sensors and chips can used measure biological signals, on the
opposite direction, live biological components can also be exploited for to power or
control different machines. Such bio-hybrid systems can be a promising solution to
build artificial creatures.
Biohybrid Systems
Multiscale Integrative NeuroEngineering Laboratory
City Unive rsity of Hong Kong
香港城市大
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La b M e mb ers
Pr incip le In ves t iga tor
Dr. Shi is an Associate Professor in the Department of Mechanical
and Biomedical Engineering at City University of Hong Kong. He
obtained his B.S. in Electrical Engineering at Wuhan University
(China), Ph.D. in Biomedical Engineering at Columbia University.
He completed his postdoctoral work at MIT in Electrical Engineering
and Biological Engineering, where he was a Simons postdoctoral
fellow. Since October 2011, he has been a faculty member of
CityU’s MBE department.
Dr. Shi, Peng Ph.D.
City Univ. | CityU Engineering | CityU MBE |
Do cto ral Students
Xu, Zhen
MS in Biomedical Engineering
Peking Union Medical College
2011
zhen.endeavor@gmail.com
Postdo cto ra l Fe llo w
Wang, Yin g
MS in Biomedical Engineering
Beihang University 2008
beatayingwang@gmail.com
Resea rch Staf fs
Lin, Xudong
PhD in Biomedical Engineering
City University of Hong Kong, 2017
Un derg radu ate Studen ts
Xie, Kai
BE in Microelectronics
Sun Yat-sen University 2013
kaixie2-c@my.cityu.edu.hk
Alumni
Li Wei (Ph.D.) Postdoctoral Associate, Georgia Institute of Technology
Amol Jadhav
Yu Xudong, PhD student at National University of Singapore
NARANG Ankit, Undergraduate student at IIT Delhi
Huang Junzhe, Research staff at the Chinese University of Hong Kong
Bao Yan, PhD student, University of Strathclyde, UK
Wang Shiqi,
Chan Chung Yuen (Joe),
Wang Chong,
After MINE-lab ...
Zh ang, Wen cong
MS in Neurosurgery
Wuhan University 2012
rrqsdsj@163.com
Xu, Bingzh e
MS in Environmental
Engineering
Northeast University 2013
xbzprince@gmail.com
Wang, Z ixun
BS in Material Chemistry
Nankai Univeristy 2014
wangzxhnb@gmail.com
Yue, Hai bin g
MS in Biology, 2015
Institute of Biophysics
Chinese Academy of Science
Multiscale Integrative NeuroEngineering Laboratory
linxud@hotmail.com
Lu , Q ian g
BE in Mechanical Engineering
University of Science and
Technology of China 2015
qianglu3-c@my.cityu.edu.hk
Zh ao, Xi
MS in Pharmacy
Nanjing University 2016
zhaoxi515@gmail.com
Ji, X ian gl in
BE In Material Science
Northwestern Polytechnical
University
jixianglinnwpu@163.com
Lu o , Xu an
BS in Opto-Information
Huazhong University of
Science and Technology 2015
x.l@my.cityu.edu.hk
Fan g , Pei l in
BE in Electronic Science and
Technology
Huazhong University of Science
and Technology 2016
plfang2-c@my.cityu.edu.hk
Kan g, Lei
BS in Mechanical Engineering
Chongqing University 2017
Kangleidada@gmail.com
Wang, Yu an
MS in Materials Science and
Engineering
Zhejiang University 2017
serancc98@gmail.com
P6423, Yeung Kin Man Academic Building
Department of Mechanical and Biomedical Engineering
City University of Hong Kong
83 Tat Chee Ave
Kowloon, Hong Kong
Tel: (852) 3442 9529
Fax: (852) 3442 0172
Email: pengshi@cityu.edu.hk
Laboratory (entrance at Lift 4)
Y1411, Academic 1
Lab tel: (852) 3442 4048
City Unive rsity of Hong Kong
香港城市大
hbyue2-c@my.cityu.edu.hk
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Pu bl i cat io ns (*denotes corresponding author)
1. “Core–Shell–Shell Upconversion Nanoparticles with Enhanced Emission for Wireless Optogenetic Inhibition”.
Lin Xudong, Chen Xian, Zhang Wenchong, Liao Qinghai, Fang Peilin, Chen Xi, He Jufang, Liu Ming, Wang
Feng*, Shi Peng*. Nano Letters, 2018,doi: 10.1021/acs.nanolett.7b04339. [pdf]
2.“Regeneration of Cortical Tissue from Brain Injury by Implantation of Defined Molecular Gradient of
Semaphorin 3A”. Zhen Xu, Wei Wang, Yutian Ren, Wenchong Zhang, Peilin Fang, Linfeng, Huang, Xin Wang,
Peng Shi*. Biomaterials, 157:125-135, 2018. [pdf]
3.“Tetherless Near-infrared Control of Brain Activity in Behaving Animals Using Fully Implantable Upconversion
Microdevices”. Ying Wang, Xudong Lin, Xi Chen, Xian Chen, Zhen Xu, Wenchong Zhang, Qinghai Liao, Xin
Duan, Xin Wang, Ming Liu, Feng Wang, Jufang He, Peng Shi*. Biomaterials, 142, 136-148, 2017. [pdf] [Movie
1, Movie 2, Movie 3, Movie 4]
4.“Multiplexed Optogenetic Stimulation of Neurons with Spectrum-Selective Upconversion Nanoparticles”.
Xudong Lin, Ying Wang, Xian Chen, Runhuai Yang, Zixun Wang, Jingyu Feng, Haitao Wang, King W. C. Lai,
Jufang He, Feng Wang*, Peng Shi*. Advanced Healthcare Materials, 2017, 6, 1700446. [pdf, frontispiece
highlight]
5.“Cell Generator: A Self-sustaining Bio-hybrid System Based on Energy Harvesting from Engineered Cardiac
Micro-tissues”. Xu Bingzhe, Lin Xudong, Li Wei, Wang Zixun, Zhang Wenchong, Shi Peng*. Advanced
Functional Materials, 2017, 27, 1606169. [pdf, frontispiece highlight, covered by nanowerk]
6. “Anti-counterfeiting Patterns Encrypted with Multi-Mode Luminescent Nanotaggants”. Tianying Sun, Bingzhe
Xu, Bing Chen, Xian Chen, Mingyu Li*, Peng Shi*, and Feng Wang*. Nanoscale, 2017 Feb 23;9(8):2701-2705.
[pdf]
7. “Investigation of the Subcellular Neurotoxicity of Amyloid-β Using a Device Integrating Microfluidic
Perfusion and Chemotactic Guidance”. W Li, Z Xu, B Xu, CY Chan, X Lin, Y Wang, G Chen, Z Wang, Q Yuan,
G Zhu, H Sun, W Wu, and P Shi*. Advanced Healthcare Materials, 2017, DOI:10.1002/adhm.201600895. [pdf]
8. “Mechanically Resilient, Injectable, and Bioadhesive Supramolecular Gelatin Hydrogels Crosslinked by Weak
Host-Guest Interactions Assist Cell Infiltration and In Situ Tissue Regeneration”. Xudong Q Feng, K Wei, S Lin,
Z Xu, Y Sun, P Shi, G Li, L Bian. Biomaterials, 101:217–228, 2016.
9. “Controllable 3D alginate hydrogel patterning via visible-light induced electrodeposition”. G Dai, W Wan, Y
Zhao, Z Wang, W Li, P Shi, Y Shen. Biofabrication, 8 (2), 025004 2016.
10.“Autonomous system for cross-organ investigation of ethanol-induced acute response in behaving larval
zebrafish”. Xudong Lin, Vincent Li, Siya Chen, Chung-Yuen Chan, Shuk-Han Cheng and Peng Shi*.
Biomicrofluidics, 10, 024123, 2016. [pdf] [Featured on Biomicrofluidics facebook ].
11.“Controlled nanoparticle release from stable magnetic microbubble oscillations”. Yu Gao, Chon U Chan,
Qiushi Gu, Xudong Lin, Wencong Zhang, Chen Loong Yeo, Astrid Marlies Alsema, Manish Arora, Seow
Khoon Chong, Peng Shi, Claus-Dieter Ohl and Chenjie Xu. NPG Asia Materials, 8, e260, 2016. [pdf].
12.“Ultrathin MXene-micropattern-based Field-effect Transistor for Probing Neural Activity”. Bingzhe Xu,
Minshen Zhu, Wencong Zhang, Xu Zhen, Zengxia Pei, Qi Xue, Chunyi Zhi, Peng Shi*. Advanced Materials, 28
(17): 3333-3339, 2016. [pdf]][Featured as back cover of this issue, covered by MaterialsViewsChina].
13.“Self-Monitoring and Self-Delivery of Photosensitizer-Doped Nanoparticles for Highly Effective Combination
Cancer Therapy in Vitro and in Vivo”. Jinfeng Zhang, et al, Peng Shi, Lung-Chen Tu, Chia-Ching Chang,
Xiaohong Zhang, Xianfeng Chen, Wenjun Zhang, Chun-Sing Lee. ACS Nano, 9(10), 9741-9756, 2015. [pdf]
14.“Interrogation of Cellular Innate Immunity by Diamond-nanoneedle-assisted Intracellular Molecular Fishing”.
Zixun Wang, Yang Yang, Zhen Xu, Ying Wang, Wenjun Zhang, Peng Shi*. Nano Letters, 15(10):7058-63,
2015. [pdf]
15.“Remote Modulation of Neural Activities via Near-infrared Triggered Release of Biomolecules”. Wei Li,
Rongcong Luo, Xudong Lin, Amol D. Jadhav, Zicong Zhang, Li Yan, Chung-Yuan Chan, Xianfeng Chen, Jufang
He, Chia-Hung Chen, Peng Shi*. Biomaterials, 65, pp. 76-85, 2015. [pdf]
16.“Photoresponsive Microvalve for Remote Actuation and Flow Control in Microfluidic Devices”. Amol D.
Jadhav, Bao Yan, Rong-Cong Luo, Li Wei, Xu Zhen, Chia-Hung Chen, Peng Shi*. Biomicrofluidics, 9, 034114,
2015. [pdf]
17.“Compartmentalized Platforms for Neuro-pharmacological Research”. A.D. Jadhav, W. Li, Z. Xu, and P. Shi*.
Current Neuropharmacology, 2016;14(1):72-86. [pdf, invited review]
18.“An upconversion nanoprobe operating in the first biological window”. Qiang Ju, Xian Chen, Fujin Ai,
Dengfeng Peng, Xudong Lin, Wei Kong, Peng Shi, Guangyu Zhu and Feng Wang. J. Mater. Chem. B, 2015, 3,
3548-3555. [pdf]
19.“Large-scale Topographical Screen for Investigation of Physical Neural-Guidance Cues”. W. Li, Q. Tang,
A.D. Jadhav, A. Narang, W. Qian, P. Shi*, and S.W. Pang. Scientific Reports, 5:8644, 2015. [pdf]
20.“High-throughput Mapping of Brain-wide Activity in Awake and Drug-responsive Vertebrates”. X. Lin, S.
Wang, X. Yu, Z. Liu, F. Wang, W. T. Li, S. H. Cheng, Q. Dai and P. Shi*. Lab on a Chip, 2015, 15(3):680-9. [pdf]
[Featured as cover of this issue]
21.“Organ-targeted high-throughput in vivo biologics screen identifies vehicles for RNA delivery”. T.Y. Chang, P.
Shi*, J.D. Steinmeyer, I. Chatnuntawech, P. Tillberg, K.T. Love, P.M. Eimon, D.G. Anderson, M.F. Yanik.
Integrative Biology, 2014,6, 897-898. [pdf] [Featured as front cover of this issue, Covered by MIT News]
22.“Near-infrared Light Responsive Multi-compartmental Hydrogel Particles Synthesized through Droplets
Assembly Induced by Superhydrophobic Surface”. R. Luo, Y. Cao, P. Shi, C.H. Chen. Small, Jul 24, 2014. [pdf]
23.“Poking Cells for Efficient Vector-free Intracellular Delivery” Y. Wang, Y. Yang, L. Yan, S. Y. Kwok, W. Li, Z.
Wang, X. Zhu, G. Zhu, W. Zhang, X. Chen and P. Shi*. Nature Communications, 5: 4466, 2014. [pdf]
24.“Near-infrared light triggerable deformation-free polysaccharide double network hydrogels R.C. Luo, Z.H.
Lim, W. Li, P. Shi, C.H. Chen. Chemical Communications, 2014 May 22. [pdf]
25."NeuroArray: A Universal Interface for Patterning and Interrogating Neural Circuitry with Single Cell
Resolution". W Li
, Z. Xu
, X. Lin, R. Luo, C. Chen, P. Shi*. Scientific Reports, 2014 4:4784. doi: 10.1038/
srep04784. [pdf]
26.“Influence of engineered surface on cell directionality and motility”. Tang QY, Tong WY, Shi J, Shi P, Lam
YW, Pang SW. Biofabrication, 2014 Mar;6(1):015011. [pdf]
27.“Site-Specific Differentiation of Neural Stem Cell Regulated by Micropatterned Multicomponent Interfaces”.
Y. Wang, Z. Xu, L.C. Kam, P. Shi*. Advanced Healthcare Materials, 2014, Feb;3(2):214-20. [pdf] [Featured
as cover of this issue, Highlighted by Materials Views China]
28.Concentration-Dependent Requirement for Local Protein Synthesis in Motor Neuron Subtype-Specific
Response to Axon Guidance CuesS. Nedelec, M. Peljto, P. Shi, M.W. Amoroso, L.C. Kam, H. Wichterle.
Journal of Neuroscience, 32(4):1496-506 2012. [pdf]
29.“Synapse microarrays: identification of small-molecules that enhance synaptogenesisP. Shi, M.A. Scott, B.
Ghosh, D. Wan, Z.Wissner-Gross, R. Mazitschek, S. Haggarty, M.F. Yanik. Nature Communications, 2: 510,
2011. [pdf] [This article was in spotlight on MIT homepage on Oct 26, 2011]
30.“Large-scale plasmonic microarrays for label-free high-throughput screeningT. Chang, M. Huang, A. A.
Yanik, H. Tsai, P. Shi, S. Aksu, M. F. Yanik, H. Altug. Lab on a Chip, 11: 3596-3602, 2011. [pdf] [Front cover of
this issue]
31.“High-resolution extracellular stimulation of dispersed hippocampal culture with high-density CMOS
multielectrode array based on non-Faradaic electrodes” N. Lei, S. Ramarkrishnan, P. Shi, J Orcutt, L. Kam, K.
Shepard. Journal of Neural Engineering, 8(4) 044003, 2011. [pdf]
32.“Combined microfluidics/protein patterning platform for pharmalogical intervention of axon pathfindingP.
Shi, S. Nedelec, H. Wichterle, L. Kam, Lab on a Chip. 10(8): 1005-10, 2010. [pdf]
33.“Dynamic force generation by Neural Stem CellsP. Shi, K. Shen, S. Ghassemi, J. Hone, L. Kam. Cellular
and Molecular Bioengineering, 2 (4): 464-474, 2009. [pdf] [Front cover of this issue, Outstanding paper from 2009
BMES annual meeting]
34.“Self-aligned supported lipid bilayers for patterning cell-substrate interface” K. Shen, J. Tsai, P. Shi, L. Kam.
Journal of American Chemical Society. 131(37):13204-5, 2009. [pdf]
35.“Local presentation of L1 and N-cadherin in multicomponent, microscale patterns differentially direct neuron
function in vitroP. Shi, K. Shen, L. Kam. Developmental Neurobiology. 67(13):1765-76, 2007. [pdf]
36.“Peptide-directed binding of quantum dots to integrins in human fibroblastP. Shi, H. Chen, M.R. Cho, M.A.
Stroscio. IEEE Trans Nanobioscience. 5(1):15-9, 2006. [pdf]
37.“Semiconductor nanostructures in biological applications” D. Alexson, H. Chen, M. Cho, M. Dutta, Y. Li, P.
Shi, A. Raichura, D. Ramadurai, S. Parikh, M.A. Stroscio, M. Vasudev. Journal of Physics: Condensed
Matter.17: 637-656, 2005.
38.“Optical and electrical properties of colloidal quantum dots in electrolytic environments: using biomolecular
links in chemically-directed assembly of quantum dot networks” M.A. Stroscio, M. Dutta, D. Ramadurai, P. Shi.
Y. Li, M. Vasudev, D. Alexson, B. Kohanpour, A. Sethuraman, V. Saini, A. Raichura, J. Yang. Journal of
Computational Electronics. 4: 21-25, 2005.
39.“Tunable optical properties of colloidal quantum dots in electrolytic environments” D. Ramadurai, B.
Kohanpour, D. Alexson, P. Shi, A. Sethuraman, Y. Li, V. Saini, M. Dutta, M. A. Stroscio. IEE Proceedings in
Nanobiotechnology. 151: 189-192, 2004.
40.“Binding of semiconductor quantum dots to cellular integrins” D. Alexson, Y. Li, D. Ramadurai, P. Shi, L.
George, M. Uddin, P. Thomas, S. Rufo, M. Dutta, M.A. Stroscio. IEEE Transactions on Nanotechnology. 3: 86-
92, 2004.
Book Chapter
Compartmentalized Synapse Microarray for High-Throughput Screening” in Microfluidic and
Compartmentalized Platforms for Neurobiological Research, Biffi Emilia (Ed.), Humana Press, 2015. (Invited)
Multiscale Integrative NeuroEngineering Laboratory
City Unive rsity of Hong Kong
香港城市大
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City Unive rsity of Hong Kong
香港城市大
Multiscale Integrative NeuroEngineering Laboratory
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Co ur s es
GE1320 , Engineering Your Health
MBE2016, Introduction to Bioengineering
MBE3101, Micro & Nanotechnology for Bioengineering
MBE3102, Human Quantitative Physiology
City Univ. | CityU Engineering | CityU MBE |
City Unive rsity of Hong Kong
香港城市大
Multiscale Integrative NeuroEngineering Laboratory
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Res ou r c es
Microscopy basics
http://www.microscopyu.com/
Fluorescent filter information on IX-81 (from Chroma)
Spectra info of the filters.
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City Unive rsity of Hong Kong
香港城市大
Multiscale Integrative NeuroEngineering Laboratory